Primer Set: Broughton N

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{"data_server":"https://primer-monitor.neb.com/igvstatic","organism_slug":"sars-cov-2","organism_name":"SARS-CoV-2","reference_accession":"NC_045512.2","primer_set_display_name":"Broughton N","primer_set_name":"Broughton_N","initial_lineage":"XFG","lineage_names":{"PQ":"PQ*","ST":"ST*","SW.2":"SW.2*","RV.2.1":"RV.2.1*","SJ.1":"SJ.1*","RE.1":"RE.1*","ST.1":"ST.1*","PG.3":"PG.3*","RE.2":"RE.2*","LF":"LF*","SV":"SV*","XFZ":"XFZ*","PG.3.2":"PG.3.2*","RV.1.3":"RV.1.3*","A":"A*","RV.1.1":"RV.1.1*","PQ.2":"PQ.2*","XFZ.4":"XFZ.4*","RW":"RW*","BA.2.86":"BA.2.86*","TA.1":"TA.1*","LP":"LP*","SW.2.1":"SW.2.1*","B":"B*","XFG.5.1":"XFG.5.1*","RE":"RE*","PG":"PG*","B.60":"B.60*","RV.1":"RV.1*","XFG.4":"XFG.4*","RK.1":"RK.1*","SJ":"SJ*","RF.5":"RF.5*","XFG":"XFG*","RV.2":"RV.2*","XFG.2":"XFG.2*","XFG.6":"XFG.6*","TA":"TA*","RK":"RK*","XFZ.4.1":"XFZ.4.1*","RW.1":"RW.1*","RV":"RV*","XFG.5":"XFG.5*","SW":"SW*","RF.5.4":"RF.5.4*","RF":"RF*","RW.1.1":"RW.1.1*","all":"All"},"variant_overlaps_path":"/organisms/sars-cov-2/lineage_variants/variant_overlaps.json"}

Lineage:

XFG* (1,637 in most recent 30 days)   └ XFG.6* (8 in most recent 30 days)   └ XFG.4*   └ XFG.2*   └ XFG.5*     └ XFG.5.1* XFZ* (306 in most recent 30 days)   └ XFZ.4* (52 in most recent 30 days)     └ XFZ.4.1* (52 in most recent 30 days) RV* (230 in most recent 30 days)   └ RV.1* (170 in most recent 30 days)     └ RV.1.3* (102 in most recent 30 days)     └ RV.1.1* (68 in most recent 30 days)   └ RV.2* (60 in most recent 30 days)     └ RV.2.1* (60 in most recent 30 days) SW* (166 in most recent 30 days)   └ SW.2* (166 in most recent 30 days)     └ SW.2.1* (166 in most recent 30 days) TA* (138 in most recent 30 days)   └ TA.1* (138 in most recent 30 days) RF* (135 in most recent 30 days)   └ RF.5* (135 in most recent 30 days)     └ RF.5.4* (65 in most recent 30 days) RW* (78 in most recent 30 days)   └ RW.1* (78 in most recent 30 days)     └ RW.1.1* (78 in most recent 30 days) ST* (58 in most recent 30 days)   └ ST.1* (58 in most recent 30 days) RK* (48 in most recent 30 days)   └ RK.1* (48 in most recent 30 days) SV* (47 in most recent 30 days) SJ*   └ SJ.1* PG*   └ PG.3*     └ PG.3.2* RE*   └ RE.1*   └ RE.2* B*   └ B.60* LP* BA.2.86* A* LF* PQ*   └ PQ.2* All

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Primers:

Name	Locus	Category	Sequence
NM B1	N2	BIP	TGCGGCCAATGTTTGTAATCAG
NM B2	N2	BIP	CCAAGGAAATTTTGGGGAC
NM B3	N2	B3	GAAATTTGGATCTTTGTCATCC
NM BIP	N2	BIP	TGCGGCCAATGTTTGTAATCAGCCAAGGAAATTTTGGGGAC
NM F1	N2	FIP	TTTGATGGCACCTGTGTAG
NM F2	N2	FIP	CGCATTGGCATGGAAGTCAC
NM F3	N2	F3	AACACAAGCTTTCGGCAG
NM FIP	N2	FIP	CGCATTGGCATGGAAGTCACTTTGATGGCACCTGTGTAG
NM LB	N2	LB	ACCTTCGGGAACGTGGTT
NM LF	N2	LF	TTCCTTGTCTGATTAGTTC

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